"Staining Solutions"

Supplier: LONZA

These stains are fluorescent dyes used to detect DNA and RNA.

Supplier: Biosensis

The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Supplier: Thermo Fisher Scientific

GelCode blue safe protein stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

Supplier: Merck

Disposable bench and drawer liners protect surfaces from stains and wear.

Supplier: AAT Bioquest

CytoWatch™ Multiplexing Buffer is a buffer optimised for the multicolor flow cytometric analysis of cells.

Supplier: Biotium

Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

Supplier: Thermo Fisher Scientific

Used for detection of glycoproteins in polyacrylamide gels.

Supplier: Brady

Super chemical resistant polyester (B-488) is designed for use in slide staining processes and other laboratory identification requiring extreme chemical resistance.

Supplier: Biotium

Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin, which can be found in the cell walls of fungi, algae, and plants. In budding yeast Calcofluor White stains bud scars more strongly, because they have a higher concentration of chitin. Calcofluor White is sometimes used to identify fungal infections in medical samples.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce 6xhis protein tag stain reagent set is a fluorescent stain for preferential detection of histidine-tagged (his-tagged) recombinant fusion proteins in protein polyacrylamide gels.

Supplier: Biotium

Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

Supplier: Merck

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference.

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

Supplier: Biotium

DAPI (4′,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.

Supplier: Abnova

DAPI counterstain provides more distinct banding patterns in applications such as staining of paraffin sections nuclear DNA.