254491 Results for: "Protein+Expression+Systems"

Supplier: TriLink BioTechnologies

The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

Supplier: TriLink BioTechnologies

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.

Supplier: TriLink BioTechnologies

Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

Supplier: Rockland Immunochemicals

Freund's incomplete adjuvant

Supplier: TriLink BioTechnologies

NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.

Supplier: TriLink BioTechnologies

 BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.

Supplier: TriLink BioTechnologies

EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

Supplier: biotechrabbit

Accessories and supplements for Rapid Translation System (RTS).

Supplier: Thermo Fisher Scientific

Control tGFP mRNA, 10 µg (0,75 µg/µl in 0,1 mM EDTA)

Supplier: OZ BIOSCIENCES

SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.

Supplier: MP Biomedicals

Freund's incomplete adjuvant mixture contains mineral oil and Arlacel A.

Supplier: OZ BIOSCIENCES

AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.

Supplier: OZ BIOSCIENCES

Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).

Supplier: Thermo Fisher Scientific

Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

Supplier: OZ BIOSCIENCES

AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.

Supplier: MP Biomedicals

Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of Mycobacterium is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.

Supplier: OZ BIOSCIENCES

Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion containing 1 mg per ml heat-killed Mycobacterium tuberculosis.

Supplier: Thermo Fisher Scientific

Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

Supplier: Enzo Life Sciences

The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.

Supplier: Corning

Axygen® gel documentation systems easily capture publication quality, 16 bit TIFF images. The systems are quick to set up and have an intuitive user interface for image capture, annotation, and contrast adjustment. Images are easily saved and opened in common gel analysis software for more detailed analysis.

Supplier: Enzo Life Sciences

The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Supplier: Enzo Life Sciences

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Supplier: VWR Collection

The VWR® Basic gel documentation system offers reproducible, high resolution digital images for gel documentation. This entry level system is easy to use and allows real time images to be captured and viewed directly on a PC or laptop (not supplied). The VWR® Basic system can be illuminated using transillumination from UV, white or blue light. These flexible lighting options make the VWR® Basic suitable for capturing and viewing images from a wide range of samples.

Supplier: BUCHI

PURE chromatography systems for flash applications.

Supplier: BUCHI

C-900 PURE Essential chromatography pump system with flexible software control.

Supplier: Syngene (a division of the Synoptics group)

The G:BOX mini is a compact, multi application imaging system for accurately imaging mini and midi fluorescence and visible gels, multiplex fluorescence Western blots, stain free gels and chemiluminescent blots.