Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: G-Biosciences

A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.

Supplier: Avantor

Avantor® ACE® Method Development Kits (MDK) are designed to maximise selectivity, offering a powerful and reliable approach to UHPLC/HPLC method development. Based upon an ultra-inert, high efficiency silica, Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness.

Supplier: Avantor

Solve U/HPLC method development challenges systematically and efficiently using Avantor® ACE® columns and ChromSword 5.1 Lite method development software. The Avantor® ACE® ChromSword Method Development Kit includes six alternative selectivity Avantor® ACE® phases, a copy of ChromSword 5.1 Lite offline with a six month activation licence and a lot of technical information and support.

Supplier: Merck

All Spectroquant® test kits can be used with the Prove range of spectrophotometers and Nova 60/60A instruments. Tests can be used not only on photometers and spectrophotometer from Merck, but also on photometers and spectrophotometers from other suppliers (programming details available on request). Spectroquant® cell test kits.

Supplier: ROTRONIC INSTRUMENTS

Water activity can be measured in production or storage rooms for the inspection of bulk materials, QC checks of cheese, meat, tobacco, building materials, animal feeds, bakery products, paper, medicines, horticulture and agriculture. The sets include advanced features for the shelf life of food, pharmaceutical and cosmetic products during storage. They document the occurrence and growth of microorganisms.

Supplier: Lovibond

These kits are mainly designed for water testing (boiling, cooling and domestic water).

Supplier: Merck

All Spectroquant® test kits can be used with the Prove range of spectrophotometers and Nova 60/60A instruments. Tests can be used not only on photometers and spectrophotometer from Merck, but also on photometers and spectrophotometers from other suppliers (programming details available on request). Spectroquant® cell test kits.

Supplier: Merck

All Spectroquant® test kits can be used with the Prove range of spectrophotometers and Nova 60/60A instruments. Tests can be used not only on photometers and spectrophotometer from Merck, but also on photometers and spectrophotometers from other suppliers (programming details available on request).

Supplier: EDVOTEK

When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Agilent

Step 2: Dispersive SPE cleanup
Select the dispersive SPE kit suited to the type of food being analysed and the method you are following. In this step, an aliquot of the sample extract from step one (see QuEChERS extraction kits) is added to a 2 or 15 ml centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: Cytiva

illustra™ GenomiPhi™ V2 DNA Amplification Kit, part of the Phi29 DNA polymerase family, is for mini-scale genomic DNA preparation. The protocol is simple. A typical DNA yield of 4 to 7 µg DNA can be achieved in less than two hours with limited hands-on time. The average product length is >10 kb. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or non purified cell lysate.

Supplier: Trajan Scientific and Medical

Kits are available for 0,5 µl to 5,0 µl NanoVolume syringes.

Supplier: Corning

Pipette pack, Axygen® Axypet™, Variable volume, Axypet Bio starter Kit 2 with one AP-10, AP-100 & AP-1000 pipettor and additional free racks of Axygen pipette tips

Supplier: EDVOTEK

An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Supplier: Cytiva

MAbTrap™ is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum and cell culture supernatants. MAbTrap™ Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time consuming buffer preparation and column packing.

Supplier: G-Biosciences

In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

Supplier: EDVOTEK

When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

Supplier: Thermo Fisher Scientific

These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

Supplier: Thermo Scientific

Thermo Scientific™ Megafuge ST1/ST1R Plus bench top centrifuges offer intuitive controls and versatility for power at every turn.

Supplier: Avantor

Avantor® ACE® Method Development Kits (MDK) are designed to maximise selectivity, offering a powerful and reliable approach to UHPLC/HPLC method development. Based upon an ultra-inert, high efficiency silica, Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: Avantor

Avantor® ACE® Method Development Kits (MDK) are designed to maximise selectivity, offering a powerful and reliable approach to UHPLC/HPLC method development. Based upon an ultra-inert, high efficiency silica, Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness.

Supplier: Avantor

Avantor® ACE® Method Development Kits (MDK) are designed to maximise selectivity, offering a powerful and reliable approach to UHPLC/HPLC method development. Based upon an ultra-inert, high efficiency silica, Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness.

Supplier: Avantor

Avantor® ACE® Method Development Kits (MDK) are designed to maximise selectivity, offering a powerful and reliable approach to UHPLC/HPLC method development. Based upon an ultra-inert, high efficiency silica, Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness.

Supplier: Hach

This kit is used for controlling boiler and cooling water treatment and checking corrosion using the drop count test which is a simple volumetric/ titration method with colour indication. It is an economical solution for the small boiler operator who needs to monitor alkalinity (5 to 400 mg/L), chloride (5 to 400 mg/L), hardness (0 to 2500 mg/L), iron (0 to 4 mg/L), and pH (4 to 10).

Supplier: Merck

All Spectroquant® test kits can be used with the Prove range of spectrophotometers and Nova 60/60A instruments. Tests can be used not only on photometers and spectrophotometer from Merck, but also on photometers and spectrophotometers from other suppliers (programming details available on request).

Supplier: Lovibond Tintometer

The Lovibond® System 3000 are a series of single-scale, 3-field instruments for visually determining the colour of samples by direct comparison with coloured glass filters. With a 3-section field of view, the sample and two consecutive glasses on the colour scale are viewed simultaneously, making it easier to achieve the optimum colour match. For rapid colour grading within pre-determined colour limits, the glasses can be set to the two limiting colours, making it easy to see if the sample is within tolerance.