Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Supplier: Merck Millipore (Novagen)

Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: Merck Millipore (Novagen)

The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Supplier: Merck Millipore (Novagen)

Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: Merck Millipore (Novagen)

Insect GeneJuice® Transfection Reagent is formulated to maximise transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for co-transfection of transfer plasmids with linearised virus DNA for the production of recombinant baculoviruses.

Supplier: Merck Millipore (Novagen)

THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Supplier: Merck Millipore (Novagen)

Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

Supplier: Merck Millipore (Novagen)

NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and recA and endA mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Supplier: Merck Millipore (Novagen)

The 10 mM dNTP mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 10 mm each in sterile deionised water at pH 7,0. Qualified for any application that requires pure deoxynucleotides, such as PCR, cDNA synthesis, and fill-in reactions.

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Supplier: Merck Millipore (Novagen)

BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

Supplier: Merck Millipore (Novagen)

BACMAGIC DNA KIT 1 * 1 KIT

Supplier: Merck Millipore (Novagen)

Rosetta-Gami 2(De3)Placi Competent Cells 1 * 1 items

Supplier: Merck Millipore (Novagen)

GRTeway® Nova pCOLA-2-DEST! DNA 1 * 10 µG

Supplier: Merck Millipore (Novagen)

LB AGAR,MILLER 1 * 25 items

Supplier: Merck Millipore (Novagen)

Ptk-neo dna 1 * 1 items

Supplier: Merck Millipore (Novagen)

Bacteriophage Ce6* 1 * 10 mL

Supplier: Merck Millipore (Novagen)

Hms174(De3)Plyss Cc* 1 * 0,4 mL