10112 Results for: "M+I+MATERIALS"
Experimentation kits, PCR-based Alu-Human DNA typing
Supplier: EDVOTEK
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.
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Kits: family pedigree cancer gene detection, Ready-to-Load™
Supplier: EDVOTEK
In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.
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Base materials
Supplier: US Biological
Casamino acids, molecularbiology grade, 500 g
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Experimentation kits, mitochondrial DNA analysis using PCR
Supplier: EDVOTEK
The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.
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Experimentation kits, exploring biotechnology with green fluorescent protein (GFP)
Supplier: EDVOTEK
Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
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Experimentation kits, bacterial culture and growth study
Supplier: G-Biosciences
This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.
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Standard prismatic optical bench, DidaFirst
Supplier: DIDALAB
Economical, recent design of prismatic optical bench
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Diode laser, green, Class II and IIIa
Supplier: DIDALAB
Extremely luminous beam, ideal for classroom experiments. Class IIIa (5 mW Power)
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Experimentation kits, antibiotic sensitivity and bacteria screening
Supplier: G-Biosciences
Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
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Prisms
Supplier: DIDALAB
Prism made of standard optical glass with index near to 1,52. Equilateral dispersing prisms are used for wavelength separation applications. Intended for demonstration experiments of light dispersion.
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Experimentation kits, antigen-antibody interactions
Supplier: G-Biosciences
This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.
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Experimentation kits, purification of the restriction enzyme Eco RI
Supplier: EDVOTEK
In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
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Lens, wide diameter, Magoptic
Supplier: DIDALAB
These wide diameter lenses are used for easy classroom experiments and for aberrations underscoring.
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Experimentation kits, determination of protein molecular weight (polyacrylamide-based)
Supplier: EDVOTEK
Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
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What does DNA look like?
Supplier: EDVOTEK
This Lab activity shows students what real chromosomal DNA Looks Like and allows them to explore the procedures involved in DNA extraction.
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Purification and size determination of green and blue fluorescent proteins
Supplier: EDVOTEK
When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
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Kits: detection of mad cow disease, Ready-to-Load™
Supplier: EDVOTEK
Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.
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VWR®, Spill Kit Chemical, 16 L
Supplier: VWR Collection
This portable, economy spill kit comes in a high visibility, water-resistant, yellow PVC bag with handles. Designed for small spills of chemicals and hazardous liquids. Ideal for small storage areas, thanks to its compact size.
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VWR®, Economy Spill Kit
Supplier: VWR Collection
These economy spill kits combine absorbents and instructions in a space-saving container. They are ideal for workers on the go like truck drivers, field workers, etc.
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VWR® SPILL-X® Spill Kits, Mobile
Supplier: VWR Chemicals
Developed to economically treat small spills, such as those that can occur in a laboratory environment. Provides easy access to the appropriate control agent for a particular type of chemical spill.
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Ready-to-Load™ Kits, what is PCR and how does it work?
Supplier: EDVOTEK
This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.
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Linking food science to biotechnology kit
Supplier: EDVOTEK
Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.
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Experimentation kits, DNA fingerprinting using restriction enzymes
Supplier: EDVOTEK
This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
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Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes
Supplier: EDVOTEK
The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
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Kits: why do people look different?, Ready-to-Load™
Supplier: EDVOTEK
This kit is for teaching students how people's physical traits are a reflection of their genes. In this simulation, students use electrophoresis to separate dyes which represent genetic traits.
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Experimentation kits, ion exchange chromatography
Supplier: EDVOTEK
Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.
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Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™
Supplier: EDVOTEK
Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.
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Experimentation kits, ion exchange chromatography
Supplier: G-Biosciences
Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.
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Drum spill kits
Supplier: Brady
The drum spill kit is designed for medium sized spills.
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Spill kaddie
Supplier: Brady
iThis is a compact wheeled spill kit for fast spill response or maintenance jobs - for chemicals and hazardous liquids.