"Ion Exchange Column"

Supplier: OriGene

FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

Supplier: VARIAN

PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation exchange functionality. The manufacturing process is controlled to provide optimum density of strong cation-exchange moieties for analysing, separating, and purifying a wide range of biomolecules from small peptides to large proteins.

Supplier: VARIAN

The PL-SAX media are well suited for anion exchange HPLC separations of proteins, peptides, and deprotected synthetic oligonucleotides. Denaturing conditions of temperature, organic solvent, and high pH levels are used for the oligonucleotide separations.

Supplier: Cytiva

HiScreen™ SP HP columns are prepacked with SP Sepharose™ High Performance ion exchange chromatography media of 4,7 ml volume and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Supplier: VARIAN

Bond Elut™ Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenone analysis. Results are comparable or superior to competing methods, including immunoaffinity columns (IaC) and charcoal/alumina columns. The sorbent is a proprietary silica-based ion exchange material.

Supplier: Thermo Fisher Scientific

These columns offer a unique HILIC selectivity and low ion exchange activity. Based on Core Enhanced Technology, they provide fast, high resolution separations without the elevated back pressures required by sub-2 µm particles.

Supplier: Resonac

SP-825, CM-825: Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. Usable across wide pH range between 2 and 12.

Supplier: Resonac

Compared to IEC series columns, polyvinyl alcohol is used as a base material offering a different separation pattern.

Supplier: Resonac

QA-825, DEAE-825: Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. Usable across wide pH range between 2 and 12.

Supplier: Avantor

Avantor® Partisil® was one of the first commercially available irregular silicas. A large surface area gives it a high loading capacity. Avantor® Partisil® ion-exchange materials are widely referenced and remain one of the most popular choices for analysts.

Supplier: EDVOTEK

In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

Supplier: Cytiva

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange resin. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Supplier: Cytiva

HiTrap Capto DEAE is a weak anion exchanger prepacked with BioProcess Capto resins for screening and small-scale protein purifications using ion exchange chromatography (IEX)..

Supplier: Cytiva

HiTrap Q XL are strong anion exchangers prepacked with Q Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Supplier: Cytiva

HiPrep™ CM FF 16/10 is a weak anion exchanger prepacked with CM Sepharose™ Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.