DST (Disuccinimidyl tartrate)
Supplier: G-Biosciences
Disuccinimidyl tartrate Homobifunctional Cross Linker.
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Sodium periodate
Supplier: G-Biosciences
G-Biosciences' Sodium meta -Periodate is a mild oxidizing agent that converts carbohydrates to activated active aldehydes
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Protein assay kits, RED 660™
Supplier: G-Biosciences
RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.
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Protease inhibitor cocktail, TCM-ProteaseArrest™
Supplier: G-Biosciences
TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.
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T4 UvsX DNA Recombinase
Supplier: G-Biosciences
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. E.coli cells expressing recombinant T4 UvsX gene.
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Magnesium sulphate 1 mol/l in aqueous solution
Supplier: G-Biosciences
Magnesium sulphate 1 mol/l in aqueous solution
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T4 UvsY Protein (from E. coli)
Supplier: G-Biosciences
T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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Human recombinant caspase 2
Supplier: G-Biosciences
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
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Electroelution device for extracting nucleic acids and proteins, GeneCAPSULE™ (G-CAPSULE™)
Supplier: G-Biosciences
G-Biosciences' GeneCAPSULE™ is an electro-elution tool for rapid, nearly 90% recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels
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TCEP-HCl (Tris(2-carboxyethyl)phosphine hydrochloride)
Supplier: G-Biosciences
TCEP-HCl (Tris(2-carboxyethyl)phosphine hydrochloride)
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Ammonium peroxodisulphate (APS) 150 mg, tablets for electrophoresis
Supplier: G-Biosciences
G-Biosciences offers Ammonium Persulfate (APS), the catalyst for the polymerization of polyacrylamide gels, in the form of ready-to-use tablets (100 count) or as a powder (100 g)
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Fraction-FOCUS™
Supplier: G-Biosciences
Fraction-FOCUS™ utilises proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins. Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.
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Bsu DNA Polymerase, Large fragment (from E. coli)
Supplier: G-Biosciences
Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. It also lacks the 3'→ 5' exonuclease activity.
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LabSafe™ Nucleic acid stain (10,000X), G-Biosciences
Supplier: G-Biosciences
LabSafe™ Nucleic Acid Stain is a safe nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels.
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Swift™ membrane stain
Supplier: G-Biosciences
Swift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and other commercially available protein stains.
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
Supplier: G-Biosciences
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
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3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) 5% in aqueous solution
Supplier: G-Biosciences
3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) 5% in aqueous solution
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Proteome kits, mammalian proteome, FOCUS™
Supplier: G-Biosciences
Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
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Lysates, mammalian GenLysate™, rat
Supplier: G-Biosciences
GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)
Supplier: G-Biosciences
A DNase and RNase free solution of 0.5M EDTA.
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Lysates, mammalian GenLysate™, human
Supplier: G-Biosciences
GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
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EDTA disodium salt dihydrate
Supplier: G-Biosciences
EDTA disodium salt is a reversible metalloprotease inhibitor that is used for chelating metal ions.
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Albumin removal kit, AlbuminOUT™
Supplier: G-Biosciences
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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Tris Buffers, Sterile
Supplier: G-Biosciences
Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.