"Biotium"

Supplier: Biotium

Dithiothreitol (DTT, Cleland's reagent), white, solid

Supplier: Biotium

EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride)

Supplier: Biotium

Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

Supplier: Biotium

Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

Supplier: Biotium

Ethidium monoazide bromide (EMA) is a fluorescent nucleic acid stain with a photoaffinity label. The dye, after photolysis, binds covalently to nucleic acids. The dye has been used to footprint drug binding sites on DNA, to modify plasmid DNA, and for labeling yeast cells for leukocyte phagocytosis assays.

Supplier: Biotium

FDP (fluorescein diphosphate, tetraammonium salt, high purity) is the most sensitive fluorogenic alkaline phosphatase substrate. It is also an excellent substrate for protein tyrosine phosphatase (PTPase) and thus the substrate of choice in the high throughput screening (HTS) for PTPase inhibitor. FDP itself is colorless, but the end product of the enzymatic reaction is the green fluorescent dye fluorescein.

Supplier: Biotium

FITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines.

Supplier: Biotium

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases.Flubida-2 is membrane-permeable and thus can be delivered into cells via simple.

Supplier: Biotium

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Supplier: Biotium

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Supplier: Biotium

Fluo-3, pentaammonium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Supplier: Biotium

Fluo-3, pentapotassium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Supplier: Biotium

Fluo-3, pentasodium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Supplier: Biotium

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins.

Supplier: Biotium

Fluorescein methotrexate is useful for studying anti-metabolite resistance and spontaneous gene amplification. The dye is actively taken up by cells and binds to dihydrofolate reductase (DHFR) quantitatively.

Supplier: Biotium

Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.