3,3',5,5'-Tetramethylbenzidine free base
About this item
TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.
- Sensitive HRP Substrate: Enables high-sensitivity detection in enzyme-linked assays
- Colorimetric Detection: Produces a strong blue signal, with a yellow endpoint upon acid stop for dual-wavelength measurement
- Flexible Formulation: Free base form allows custom preparation for specific assay conditions
- Versatile Applications: Ideal for ELISA, Western blotting, and immunoassays in diagnostics and research
- Low Background: Provides high signal-to-noise ratio for accurate quantification
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Product Details & Documents
TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.
- Sensitive HRP Substrate: Enables high-sensitivity detection in enzyme-linked assays
- Colorimetric Detection: Produces a strong blue signal, with a yellow endpoint upon acid stop for dual-wavelength measurement
- Flexible Formulation: Free base form allows custom preparation for specific assay conditions
- Versatile Applications: Ideal for ELISA, Western blotting, and immunoassays in diagnostics and research
- Low Background: Provides high signal-to-noise ratio for accurate quantification
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Product Information
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