Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

• T47D cell line human has been used:
• To study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• Has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• To study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

DNA profile: STR-PCR Data: Amelogenin: X, CSF1PO: 11,13, D13S317: 12, D16S539: 10, D5S818: 12, D7S820: 11, THO1: 6, TPOX: 11, vWA: 14.

Culture medium: DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70 to 80%) 1:3 to 1:6 i.e. seeding at 2 to 4×10,000 cells/cm² using 0,25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Karyotype: 2n = 46, hypertriploid
Morphology: Epithelial
Technique: Cell culture
Relevant disease: Cancer
Synonym(s): T-47-D Cells, T-47D Cells, T47D:A Cells