Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Rat
- Target protein:NSE/ENO2
- Description:Rat NSE/ENO2 ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat NSE/ENO2 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat NSE/ENO2. No significant cross-reactivity or interference between NSE/ENO2 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:78,125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:46,875 pg/ml
- Regulatory status:RUO
Specifications
About this item
Rat NSE/ENO2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat NSE/ENO2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA Kit
- Detection range: 78,125 to 5000 pg/ml
- Sensitivity: 46,875 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat NSE/ENO2 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat NSE/ENO2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for NSE/ENO2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the NSE/ENO2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-NSE/ENO2 Antibody, which binds the captured NSE/ENO2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of NSE/ENO2 captured in each well. The concentration of NSE/ENO2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.