Mouse CCL25 ELISA Kit

Mouse CCL25 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CCL25 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse CCL25 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse CCL25 in serum, plasma or other biological fluids. An antibody specific for CCL25 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CCL25 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-CCL25 antibody, which also binds the CCL25 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-CCL25 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-CCL25 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of CCL25 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of CCL25 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.