Human Sphingomyelin Synthase 1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Sphingomyelin Synthase 1 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Detection range: 50 to 800 ng/L
• Sensitivity: 3,05 ng/L
• Sample type: Serum, plasma or other biological fluids
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human Sphingomyelin Synthase 1 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Sphingomyelin Synthase 1 in An antibody specific for Sphingomyelin Synthase 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Sphingomyelin Synthase 1 present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-Sphingomyelin Synthase 1 antibody, which also binds the Sphingomyelin Synthase 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Sphingomyelin Synthase 1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Sphingomyelin Synthase 1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Sphingomyelin Synthase 1 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Sphingomyelin Synthase 1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.