Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. It also lacks the 3'→ 5' exonuclease activity.

• Product source: E. coli strain expressing Bsu DNA polymerase, large fragment gene
• Storage temperature: −20 °C
• Heat inactivation: 65 °C for 20 min

Product includes: Bsu DNA polymerase, large fragment and 10X Bsu DNA polymerase, large fragment reaction buffer

10X Bsu DNA polymerase, large fragment:
100 mM Tris-HCl
500 mM KCl
100 mM MgCl2
10 mM DTT, pH 7,9 at 25 °C

Storage buffer:
50mM Tris-HCl
50 mM KCl
1 mM DTT
0,1 mM EDTA
50% Glycerol, pH 7,5 at 25 °C

Unit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37 °C
Quality Control assays: Bsu DNA Polymerase, Large fragment is free from detectable nuclease contamination

Applications:
Second strand cDNA synthesis
Strand displacement DNA synthesis
Random primer labeling
dA-tailing