T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.

• Product source: E. coli cells expressing T4 UvsY gene
• Storage temperature: −20 °C
• Quality control assays: UvsY is free from detectable nuclease contamination

UvsY stimulates the DNA-dependent ATPase activity of UvsX, lowers the critical concentration of UvsX that is required for activity, and promotes strand exchange. UvsY efficiently promotes the UvsX-catalysed strand invasion reaction by recruiting to ssDNA-gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.

Product includes: T4 UvsY protein and 10X UvsY reaction buffer

10X UvsY reaction buffer composition:
200 mM Tris-acetate
1M Potassium acetate
100 mM Magnesium acetate
10 mM DTT
pH 7,8 at 25 °C

Storage buffer:
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0,1 mM EDTA
50% glycerol
pH 7,5 at 25 °C