For the quantification of LTC₄ in culture media and other sample matrices.

• Measure LTC₄ in tissue culture medium
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure LTC₄ levels down to 40 pg/ml
• Incubation: 18 hours | Development: 90 to 120 minutes | Read: Colorimetric at 405 to 420 nm

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA₄ with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA₄ results in the formation of LTC₄, which is then rapidly metabolised to LTD₄ and LTE₄. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC₄ ELISA Kit is a competitive assay that can be used for quantification of LTC₄ in culture media and other sample matrices. The assay has a range from 17,1 to 5000 pg/ml and a sensitivity (80% B/B₀) value of approximately 40 pg/ml.