Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.

• Sensitive, specific assay for screening GLS1 inhibitors
• Includes hrGLS1 and CB-839 (inhibitor control)

Glutaminase (EC 3.5.1.2, GLS) is an enzyme that hydrolyses glutamine producing glutamate and ammonia. It has tissue-specific roles in multiple organs, some of which include ammonia generation for urea synthesis in the liver; maintenance of acid-base homeostasis by ammonia production during renal acidosis in the kidney; and regulation of neurotransmitter glutamate in the brain. The isoform glutaminase 1 (GLS1) or phosphate-activated mitochondrial glutaminase is critical for glutamine utilisation by cancer cells and the rate of glutaminolysis is known to increase in tumors, making it extremely important for tumor cell metabolism. GLS1 inhibitors have been considered as potential candidate drugs for cancer therapy. This glutaminase (GLS1) inhibitor screening kit is a plate-based fluorometric kit for screening human GLS1 inhibitors. The hydrolysis of glutamine by glutaminase forms glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. A well-known glutaminase inhibitor - CB-839 is provided as an inhibitor control.

All products are FOR RESEARCH USE ONLY.   PRODUCTS ARE NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  Customer is granted a limited use non-exclusive licence for its own internal research.  Any control manufacturer supplies with a Product is limited to use only to validate the relevant Product.  By using the Product, Customers agree not to attempt to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, processes, make-up or production of any of the Products or portions thereof.