Specifications

• Pk:
  1 mg
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His, Flag Tag (active trimer) (MALS verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Loaded Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 1,58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 18,76 - 19,45 ng/ml in the presence of 5 ng/ml of Recombinant Human IL‑4 (Routinely tested).
• Gene ID:
  P29965-1
• Protein synonyms:
  TRAP|CD40L|IMD3|gp39|CD154|CD40LG|IGM|T-BAM|TNFSF5|HIGM1
• Protein/peptide name:
  CD40 Ligand
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular weight:
  49,9 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before Lyophilisation.
• Shipping temperature:
  RT