Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Paguma larvata
• Tag sequence:
  His Tag (SPR verified)
• Storage conditions:
  –70 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) Immobilised on CM5 Chip can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 0,101 μM as determined in a SPR assay (Biacore T200) (QC tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) Immobilised on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 29 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) Immobilised on CM5 Chip can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 47,5 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) Immobilised on CM5 Chip can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 0,796 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) with an affinity constant of 1,17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q56NL1-1
• Protein synonyms:
  ACEH|ACE-2|ACE2
• Protein/peptide name:
  ACE2
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular weight:
  85,7 kDa
• Endotoxin level:
  Low
• Formulation:
  Delivered as bulk protein in a 0,2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7,5 with glycerol as protectant.
• Shipping temperature:
  Dry Ice