Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 0,01 EU per μg by the LAL method.
• Biological activity:
  Lyopilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin at 5 μg/ml (100 µl/well) can bind Anti-CD33, Human IgG1 with a linear range of 0,078-1,25 μg/ml (QC tested).; Loaded Siglec-3 MAb (Mouse IgG1) on AMC Biosensor, can bind Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin with an affinity constant of 0,365 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P20138-1(R69G)
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  gp67|SIGLEC3|CD33
• Protein/peptide name:
  Siglec-3
• Purity:
  >95%
• Molecular weight:
  54,6 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyopilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT