Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised human latent TGFB1, His Tag (Cat. No. ACRBTG1-H524x) at 5 μg/ml (100 μl/well) can bind biotinylated human ITGAV and ITGB6 heterodimer protein, His,Avitag and Tag Free (Cat. No. ACRBIT6-H82E4) with a linear range of 1 - 20 ng/ml (QC tested).; Measured by its binding ability in a functional ELISA. Immobilised human latent TGFB1, His Tag (Cat. No. ACRBTG1-H524x) at 5 μg/ml (100 μl/well) can bind human LRRC32, Fc Tag (Cat. No. ACRBLR2-H5256) with a linear range of 0,039 - 0,625 μg/ml (routinely tested). Human latent TGFB1, His Tag (Cat. No. ACRBTG1-H524x) inhibits the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 0,293 - 0,406 ng/ml (routinely tested).
• Gene ID:
  P01137-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  CED|TGF-beta-1|TGFB|DPD1|Latent TGF-beta 1|Latent TGFB1|LAP|TGFB1
• Protein/peptide name:
  Latent TGF-beta 1
• Purity:
  >95%
• Molecular weight:
  42,6 kDa (monomer)
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT