Specifications

• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised natalizumab at 1 μg/ml (100 μl/well) can bind biotinylated human ITGA4 and ITGB1 heterodimer protein, His,Avitag and tag free (Cat. No. ACRBIT1-H82W1) with a linear range of 2 - 31 ng/ml (QC tested).; Measured by its binding ability in a functional ELISA. Immobilised Human VCAM-1, His Tag (Cat. No. ACRBVC1-H5224) at 5 μg/ml (100 μl/well) can bind biotinylated human ITGA4 and ITGB1 heterodimer protein, His,Avitag and tag free (Cat. No. ACRBIT1-H82W1) with a linear range of 0,01 - 0,156 μg/ml (routinely tested).
• Gene ID:
  P13612-1 (ITGA4) and P05556-1 (ITGB1)
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  Integrin alpha 4 beta 1|ITGA4 and ITGB1
• Protein/peptide name:
  Integrin alpha 4 beta 1
• Purity:
  >95%
• Molecular weight:
  113,2 kDa (ITGA4) and 83,7 kDa (ITGB1)
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT