Aviva Systems Biology Aflatoxin Total ELISA Kit (OKAO00139) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Aflatoxin Total has been pre-coated onto a 96-wellplate (12 × 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Aflatoxin Total antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Aflatoxin Total in the sample.