Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

• Detect phagocytosis of fluorescein-labelled rabbit IgG-coated latex beads​
• Validated in human peripheral blood monocytes and mouse peritoneal macrophages
• Phagocytosis detectable in 1 to 4 hours at 37 °C
• Assess by flow cytometry or fluorescence microscopy

Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labelled rabbit IgG. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualising perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough latex beads-rabbit IgG-FITC complex for up to 750 samples.