A competitive assay for measurement of enterolactone in plasma, urine, and other biological sample matrices.

• Measure enterolactone in plasma, urine, and other sample matrices
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure enterolactone levels down to 70 pg/ml
• Colorimetric reading at 405 to 420 nm

Enterolactone is a mammalian lignan with an estrogen-like diphenolic structure. It is produced by intestinal bacteria from two plant precursors (matairesinol and secoisolariciresinol) obtained in the diet. Enterolactone and other lignans and phytoestrogens have been associated with a reduced risk of acute coronary events, hormone-dependent cancers, and possibly osteoporosis. Several studies have suggested that serum enterolactone may serve as a biomarker of a healthy, high-fiber diet. Cayman Chemical’s Enterolactone ELISA kit is a competitive assay that utilizes a standard curve ranging from 15,6 to 2000 pg/ml. The assay has a range from 15,6 to 2500 pg/ml and a sensitivity (80% B/B₀) of approximately 70 pg/ml.