Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®
SIALP7059-1L
: Merck
:
- Pk:1 L
- Description:10X Phosphate-Buffered Saline (PBS)
- pH value:7,2 - 7,6
Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
Dilute 10X PBS to a 1X working solution using molecular biology grade water.
It contains 30 mM sodium phosphate, 1,5 M sodium chloride and 10,5 mM potassium phosphate.