The PC-12 cell line was derived from a transplantable rat adrenal pheochromocytoma. These cells were maintained in Ham's F12K medium with L-glutamine (2 mM) adjusted to contain 1,5 g/L sodium bicarbonate (82,5%), horse serum (15%) and faetal bovine serum (2,5%).

Liquid lysates in SDS-PAGE sample buffer, pH 6,8, heated for 10 minutes at 70 °C and containing Tris-HCl (40 mM), SDS (1%), DTT (50 mM), glycerol (7,5%), and bromophenol blue (0,003%).