For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

The core kit contains unique formulations of buffers and reagents that allow isolation of mitochondria and cytosol in only two easy steps. No ultracentrifugations are required. No toxic chemicals are involved.