CD3⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

• Purity of >90% by flow cytometry
• Cryopreserved in CryoStor® CS10 media
• Test negative for Hepatitis B, Hepatitis C, HIV 1/2, HTLV-I/II, HIV-1/HCV/HBV nucleic acid, WNV nucleic acid, Trypanasoma cruzi and Syphilis

The CD3⁺ T cell possesses a complex of cell surface molecules that associates with the T-cell antigen receptor (TCR). CD3 functions in the cell surface expression of TCR and in the signaling transduction cascade that originates when a peptide: MHC ligand binds to the TCR. CD3 surface protein is made of invariant subchains belonging to the immunoglobulin superfamily. CD3 is found on the surfaces of T cells and of thymocytes. Mutant expression of the TCR can be found in Severe Combined Immunodeficiency (SCID), and monoclonal antibodies to the CD3 marker have been demonstrated to be an effective way to control allografts by preventing T-cell activation.

Storage: Either prepare cells for long term storage in liquid nitrogen vapour phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at –80 °C is acceptable, but should be minimised to ensure maximum stability. Once thawed, samples must be used immediately.