Fluorometric polymerase activity assay kit, EvaEZ™

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

• Just add polymerase
• No radioisotopes needed
• Can be used at temperatures from 4 to 75 °C

EvaEZ™ Fluorometric Polymerase Activity Assay Kit contains EvaGreen® dye together with a primed template, dNTPs, and MgCl₂ in a Tris buffer system.

In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase.

The EvaEZ™ Fluorometric Polymerase Activity Assay was developed for the measurement of Taq DNA polymerase activity but can also be used for other DNA polymerases, such as Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I.