Pierce™ Ni-NTA spin columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

• High capacity binding up to 60 mg of 6xHis-tagged protein per milliliter of resin
• Purify proteins using native or denaturing conditions
• Resin can be reused at least five times without reduction in performance
• Purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates

The specially prepared support consists of beaded agarose derivatised with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni²⁺). The immobilised metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.