- Antibody type:Primary
- Antigen name:Neurofilament Medium
- Clonality:Monoclonal
- Clone:3H11
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1
- Reactivity:Pig,Chicken,Human,Bovine,Rat,Mouse,Cat
- Western blot:Yes
- Environmentally Preferable:
- Cross adsorption:No
- Format:Lyophilized
- Antigen synonyms:Neurofilament medium polypeptide|Nfm,
- Immunogen:Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
- Size:100 µl
- Pk:100 µl
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Specifically recognizes the medium neurofilament subunit NF-L in WB.
Application Information:
Western Blotting (WB), Immunocytochemistry (IC) and Immunohistochemistry (IH). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Neurofilament Medium
Clonality: Monoclonal
Clone: 3H11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Rat, Mouse, Cat, Bovine, Pig, Chicken