The GlycanPac™ AXR-1 column uses volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile, presenting the eluting glycans ready for introduction into MS instruments. Designed and tested for HPLC and UHPLC methods using either fluorescence or MS detection applications. Ultra-high resolution and selectivity of glycans are attributed to the GlycanPac™ AXR-1 column's innovative mixed-mode surface chemistry, which combines:

• WAX functionality for retention of negatively charged glycans, with separation in order of increasing charge
• Reversed-phase functionality for same-charge glycans, with separation according to isomerism and size

Particle type: high purity, spherical, porous silica

Bonded phase: mixed-mode: weak anion exchange and reversed phase

pH range: 2,0 to 8,0