Multimode columns, HiTrap™ Capto™ adhere

HiTrap Capto adhere is prepacked with Capto adhere, a multimodal BioProcess ion exchange resin designed to remove contaminats in post-protein A purification.

• High capacity and productivity in flowthrough mode
• Savings in time and operating costs with a two-step chromatographic process
• Wide operational window of pH and conductivity
• Prepacked HiTrap columns for easy screening and convenient process development

Capto adhere is a strong anion exchanger with multimodal functionality. The multimodal functionality gives a different selectivity compared to traditional anion exchangers. Capto adhere is designed for post-protein A purification of monoclonal antibodies.

Removal of leached protein A, aggregates, host cell proteins, nucleic acids, and viruses from monoclonal antibodies is performed in flowthrough mode at which the antibodies pass directly through the column while the contaminants are adsorbed. For optimal performance of Capto adhere, screening/optimization of loading conditions are required.