Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Charles River Laboratories Cell Solutions

Mononuclear cells (MNCs) used for researching the causes, diagnosis, supportive care and treatment of Hepatitis C Virus (HCV). Human mononuclear cells are collected from human volunteer donors diagnosed with Hepatitis C virus under IRB approved informed consent.

Supplier: ENZO LIFE SCIENCES

The PC-12 cell line was derived from a transplantable rat adrenal pheochromocytoma. These cells were maintained in Ham's F12K medium with L-glutamine (2 mM) adjusted to contain 1,5 g/L sodium bicarbonate (82,5%), horse serum (15%) and faetal bovine serum (2,5%).

Supplier: PROVITRO

B cells have been isolated from mononuclear cells through either positive or negative selection using immunomagnetic cell separation techniques.

Supplier: Charles River Laboratories Cell Solutions

CD3⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M1 macrophages are derived from purified monocytes. CD14⁺ monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures and were cultured in the presence of GM-CSF for 10 days. The M1 phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: PROVITRO

Dental Pulp Stem Cells (DPSCs) are primarily sourced from the pulp tissues of deciduous teeth, primary incisors, and permanent third molars. The dental pulp, especially from third molars, is noted for its rich reservoir of stem cells. DPSCs derived from third molars express key pluripotency-associated transcription factors including oct4, sox2, klf4, nanog, and c-myc.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Charles River Laboratories Cell Solutions

CD34⁺ cells are isolated using positive immunomagnetic cell separation procedures from bone marrow. Bone marrow aspirates and cells, drawn from the posterior iliac crest using a syringe containing anticoagulant, are collected from normal human volunteers using institutional review board (IRB) approved procedures.

Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: PROVITRO

HCASMC - Human coronary artery smooth muscle cells - are obtained from human tissue, in particularly from the coronary artery smooth muscle cells, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation.

Supplier: PROVITRO

CD14⁺ T cells, obtained from mononuclear cells, are isolated through either negative or positive selection using immunogenetic cell separation techniques. These cells play a crucial role in the immune response against infections and are valuable for the investigation and diagnosis of immune reactions, particularly in infections and inflammation.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells. These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.

Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: Charles River Laboratories Cell Solutions

CD56⁺ NK cells are isolated from mononuclear cells either by positive selection using an antibody that targets the CD56 antigen, or by negative selection using immunomagnetic cell separation procedures.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

HKER-d - Human keratinocytes from dermis - are obtained from human tissue, in particularly from the Dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HKER cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: ENZO LIFE SCIENCES

Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0,5 µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay.

Supplier: Charles River Laboratories Cell Solutions

The mononuclear cell is a widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Charles River Laboratories Cell Solutions

Neutrophils are derived from a dextran red cell sedimented population of cells. Following red cell sedimentation, granulocytes are separated using a density gradient and red cell lysis. Cells are then purified through the use of indirect selection.

Supplier: PROVITRO

HUAEC - Human Umbilical Artery Endothelial Cells) are obtained from human tissue, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUAEC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

HPASMC - Human pulmonary artery smooth muscle cells - are obtained from human tissue, in particularly from the pulmonary artery , initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HPASMC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: PROVITRO

HUASMC - Human umbilical artery smooth muscle cells - are obtained from human tissue, in particularly from the umbilical artery, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUASMC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: PROVITRO

HFIB-d - Human Fibroblast from dermis - are obtained from human tissue, in particularly from the dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HFIB cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.