You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Single tissue blots, multiple species
Supplier: G-Biosciences
Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.
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Eye tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.
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Human cancer cell line blot
Supplier: G-Biosciences
Ready to screen human cancer cell line blots.
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Chemiluminescent acridinium esters
Supplier: ENZO LIFE SCIENCES
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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Kidney tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.
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Immunodetection reagents, femtoLUCENT™ PLUS
Supplier: G-Biosciences
The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.
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picoLUCENT™ PLUS-HRP Kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Cell extract HL60 (non-induced)
Supplier: ENZO LIFE SCIENCES
Whole-cell extract of human HL-60 leukemia cells.
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picoLUCENT™ PLUS-AP kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Brain tissue region blots, single species
Supplier: G-Biosciences
G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.
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Heart tissue region blots, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.
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NPP ELISA alkaline phosphatase substrate
Supplier: Rockland Immunochemicals
NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.
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Caspase-3 substrates, NucView®
Supplier: Biotium
NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.
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Botanical garden blot
Supplier: G-Biosciences
G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
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Peroxidase conjugate stabiliser/diluent, Guardian™
Supplier: Thermo Fisher Scientific
Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.
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1-Step™, Pierce™ Chloronaphthol
Supplier: Thermo Fisher Scientific
Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
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Amplite® West ECL HRP Substrate *Femto Sensitivity*
Supplier: AAT BIOQUEST
Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.
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Chemiluminescent EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.
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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™
Supplier: Thermo Fisher Scientific
QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
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CN/DAB substrate kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
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DAB substrate
Supplier: Thermo Fisher Scientific
DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
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Biotin random prime DNA labelling kit, North2South™
Supplier: Thermo Fisher Scientific
North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
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Pierce™ OPD (o-phenylenediamine Dihydrochloride)
Supplier: Thermo Fisher Scientific
OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.
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Pierce™ Firefly Signal Enhancer (100X)
Supplier: Thermo Fisher Scientific
Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.
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Chemiluminescent RNA EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
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Fluorogenic peroxidase substrate kit, QuantaBlu™
Supplier: Thermo Fisher Scientific
QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.
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Pierce™ Biotin 3' End DNA Labelling Kit
Supplier: Thermo Fisher Scientific
This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit
Supplier: Thermo Fisher Scientific
The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.