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Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Bufor do strippingu Western blot, Western ReProbe™

Bufor do strippingu Western blot, Western ReProbe™

Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

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Rozcieńczalnik do western blotting, Swift™ Western Diluent

Rozcieńczalnik do western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Podkładki transferowe do western blotting, Swift

Podkładki transferowe do western blotting, Swift

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

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Western ReProbe™ PLUS

Western ReProbe™ PLUS

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

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Pojedynczy blot tkankowy myszy, pojedynczy gatunek

Pojedynczy blot tkankowy myszy, pojedynczy gatunek

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Pojedyncze plamy tkanek szczura, pojedyncze gatunki

Pojedyncze plamy tkanek szczura, pojedyncze gatunki

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Western blots, uprzednio przygotowane, ludzka pojedyncza tkanka, pojedynczy rodzaj

Western blots, uprzednio przygotowane, ludzka pojedyncza tkanka, pojedynczy rodzaj

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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Zestawy Immunoblottingu, MaxTag™

Zestawy Immunoblottingu, MaxTag™

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

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Western blot, wstępnie przygotowany, specyficzny dla frakcji subkomórkowej myszy

Western blot, wstępnie przygotowany, specyficzny dla frakcji subkomórkowej myszy

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

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Zestaw do Western blotting, ECL substrat, Pierce™

Zestaw do Western blotting, ECL substrat, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

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Odczynniki do wykrywania Western blot, substrat ECL do Western blot, Thermo Fisher Scientific

Odczynniki do wykrywania Western blot, substrat ECL do Western blot, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Bufor do strippingu Western blot, restore PLUS™, Thermo Fisher Scientific

Bufor do strippingu Western blot, restore PLUS™, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

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Bufor do odymywania w Fluorescencji Western Blot, Restore™

Bufor do odymywania w Fluorescencji Western Blot, Restore™

Supplier: Thermo Fisher Scientific

Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

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Wzmacniacz sygnału Western blot, Pierce™, Thermo Fisher Scientific

Wzmacniacz sygnału Western blot, Pierce™, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.

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Zestawy i odczynniki do detekcji IP Clean-Blot™

Zestawy i odczynniki do detekcji IP Clean-Blot™

Supplier: Thermo Fisher Scientific

Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

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Zestaw S-nitrolizacyjny Western Blot Kit, Pierce™

Zestaw S-nitrolizacyjny Western Blot Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

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Peroksydaza chrzanowa, Pierce™

Peroksydaza chrzanowa, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

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Substrat do Western Blotting, Pierce™ ECL Plus

Substrat do Western Blotting, Pierce™ ECL Plus

Supplier: Thermo Fisher Scientific

The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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Rozszerzony roztwór przeciwciał NC, Miser™

Rozszerzony roztwór przeciwciał NC, Miser™

Supplier: Thermo Fisher Scientific

Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

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Zestaw do Far-Western Blot dla biotylowanych protein, Pierce™

Zestaw do Far-Western Blot dla biotylowanych protein, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.

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Bufor do strippingu Western blot, Restore™, Thermo Fisher Scientific

Bufor do strippingu Western blot, Restore™, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

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Wzmacniacz do Western blot, SuperSignal™, Pierce

Wzmacniacz do Western blot, SuperSignal™, Pierce

Supplier: Thermo Fisher Scientific

SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

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Bufor transferowy wysokąej masy cząsteczkowej (5X)

Supplier: G-Biosciences

High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.

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Koningaty HRP, HisProbe™

Koningaty HRP, HisProbe™

Supplier: Thermo Fisher Scientific

HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

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Zestaw West HisProbe, SuperSignal™

Zestaw West HisProbe, SuperSignal™

Supplier: Thermo Fisher Scientific

SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

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SuperSignal™ West Pico PLUS Odczynnik chemiluminescencyjny

SuperSignal™ West Pico PLUS Odczynnik chemiluminescencyjny

Supplier: Thermo Fisher Scientific

SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.

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Substrat do Western blotting, 1-Step™ NBT/BCIP

Substrat do Western blotting, 1-Step™ NBT/BCIP

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and in situ hybridisation applications.

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Roztwór 1-Step™ Ultra TMB-Blotting, Pierce™

Roztwór 1-Step™ Ultra TMB-Blotting, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

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Substrat do Western blotting, 1-Step™ NBT/BCIP plus suppressor

Substrat do Western blotting, 1-Step™ NBT/BCIP plus suppressor

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.

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Substrat do Western blotting, NBT (nitro-niebieski chlorek tetrazolu)

Substrat do Western blotting, NBT (nitro-niebieski chlorek tetrazolu)

Supplier: Thermo Fisher Scientific

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

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