Supplier: Merck Millipore (Novagen)

KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile Thermococcus kodakaraensis KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Cytiva

T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Merck

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions ofphosphoramidites during the extension step. The activator reacts with theamidite group to form a highly reactive intermediate. The intermediate thenforms an internucleotide bond with the detritylated 5′hydroxyl group of thegrowing oligonucleotide chain.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Supplier: Merck

Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.

Supplier: BIOPREMIER

This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: BIOPREMIER

SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Supplier: Cytiva

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Supplier: Thermo Fisher Scientific

ABsolute Blue™ Master Mix incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy and foolproof. Applications include gene expression, siRNA validation, SNP typing and genotyping.

Supplier: Cytiva

illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: PRIMERDESIGN

This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from Salmonella spp. The kit is designed for the in vitro quantification of Salmonella spp genomes and to have a broad detection profile.

Supplier: Merck

Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.

Supplier: Merck

PCR ReadyMix™ is intended for use with Extract-N-Amp™ Plant PCR kit and Extract-N-Amp™ Tissue PCR kit. All Extract-N-Amp kits include a PCR ReadyMix™ sufficient for one PCR reaction per extraction. However, where additional PCR reactions are required, supplemental PCR ReadyMix™ may be needed. The REDExtract-N-Amp™ PCR ReadyMix contains a inert tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.