Szukałeś: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow
Supplier: Cytiva
Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Ion exchange chromatography media, DEAE Sephacel™
Supplier: Cytiva
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
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Affinity chromatography media, Blue Sepharose™ High Performance
Supplier: Cytiva
This media is suitable for affinity chromatography.
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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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Accessories for ÄKTA chromatography systems
Supplier: Cytiva
Tubing connector, SRTC, PEEK, I.Ø 1,5 mm, 2 ports
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Affinity chromatography media, Lentil Lectin Sepharose™ 4B
Supplier: Cytiva
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Concentrator
Supplier: G-Biosciences
The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.
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Affinity purification media, GammaBind™ Plus Sepharose™
Supplier: Cytiva
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
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Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance
Supplier: Cytiva
Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
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Ion exchange chromatography media, DEAE Sepharose™ CL-6B
Supplier: Cytiva
DEAE Sepharose™ CL-6B is a weak anion exchanger.
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Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Affinity chromatography media, MabSelect™ PrismA
Supplier: Cytiva
MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.
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Ion exchange chromatography media, SOURCE™ 15S
Supplier: Cytiva
SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Gel filtration media, Sephadex™ G-75
Supplier: Cytiva
Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
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Superose™ 12 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability
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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.
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2D Clean-Up kit
Supplier: Cytiva
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow
Supplier: Cytiva
DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.
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Affinity chromatography media, Capto™ L
Supplier: Cytiva
Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
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Amicon® Pro Purification System
Supplier: MILLIPORE
The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.
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Ion exchange chromatography media, Ion Exchanger I
Supplier: Merck
Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.