You searched for: Protein Purification

Supplier: Merck Millipore (Oncogene)

Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

Supplier: Thermo Fisher Scientific

Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

Supplier: Thermo Fisher Scientific

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

Supplier: Cytiva

Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.

Supplier: Cytiva

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.

Supplier: Cytiva

Capto™ adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process.

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

Supplier: Cytiva

Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Supplier: Cytiva

Septum, silicone with PTFE backing