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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Protein A Sepharose™

Protein A Sepharose™

Supplier: Rockland Immunochemicals

Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from Staphylococcus aureaus. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

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Proteasome Purification Kit

Supplier: ENZO LIFE SCIENCES

Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

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TEV Protease Kit

Supplier: Biotium

TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

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J.T.Baker® BAKERBOND®, Supported Liquid Extraction Plates

J.T.Baker® BAKERBOND®, Supported Liquid Extraction Plates

Supplier: Avantor

J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

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Rabbit affinity purification resin

Rabbit affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Rabbit IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Rabbit IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Sheep affinity purification resin

Sheep affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Sheep IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Sheep IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Pep-Extract™

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

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Protein desalting and buffer exchange columns, SpinOUT™

Protein desalting and buffer exchange columns, SpinOUT™

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

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Proteomic protein controls

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

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Pierce™ Protein Purification Kit, Protein A IgG Purification Kit

Pierce™ Protein Purification Kit, Protein A IgG Purification Kit

Supplier: Thermo Fisher Scientific

The Protein A IgG Purification Kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilised Protein A in a buffer that facilitates binding. After non IgG components are washed from the column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column.

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HisPur™ Ni-NTA spin purification kits

HisPur™ Ni-NTA spin purification kits

Supplier: Thermo Fisher Scientific

The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.

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Protein isolation and purification, FastPROTEIN™ blue matrix kit, MP Biomedicals

Protein isolation and purification, FastPROTEIN™ blue matrix kit, MP Biomedicals

Supplier: MP Biomedicals

The FastPROTEIN™ Blue Matrix Kit (also known as Lysing Matrix B) is designed for rapid isolation and purification of protein from bacterial cultures.

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Pierce™ Protein A Agarose

Pierce™ Protein A Agarose

Supplier: Thermo Fisher Scientific

Pierce™ Protein A agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

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Pierce™ Protein G Plus Agarose

Pierce™ Protein G Plus Agarose

Supplier: Thermo Fisher Scientific

Pierce™ protein G plus agarose is a high capacity protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

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Protein L Plus agarose, Pierce™

Protein L Plus agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein L Plus agarose is a high-capacity Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

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Recombinant protein A/G, Pierce™

Recombinant protein A/G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Affinity chromatography media, glutathione superflow agarose, pierce™

Affinity chromatography media, glutathione superflow agarose, pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione Superflow Agarose consists of glutathione immobilised to a crosslinked 6% beaded agarose for purification of GST-tagged proteins.

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Immobilised NeutrAvidin™, UltraLink™, Pierce™

Immobilised NeutrAvidin™, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

NeutrAvidin™ UltraLink™ resin is a beaded polyacrylamide resin of immobilised NeutrAvidin™ Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

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Coupling Resin, AminoLink™ Plus

Coupling Resin, AminoLink™ Plus

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

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Immobilised D-galactose, Pierce™

Immobilised D-galactose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised D-Galactose agarose enables purification of lectins, galactosidases and other galactose-binding molecules. The immobilised D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.

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Protein G agarose, Pierce™

Protein G agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein G agarose is a premium quality affinity resin for antibody purification.

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Protein A/G agarose, Pierce™

Protein A/G agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G agarose is a affinity resin for antibody purification.

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Recombinant protein A, Pierce™

Recombinant protein A, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Pierce™, Melon™ IgG spin Purification Kits, Gel

Pierce™, Melon™ IgG spin Purification Kits, Gel

Supplier: Thermo Fisher Scientific

The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

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Protein L agarose, Pierce™

Protein L agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein L agarose is a premium quality Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

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Biosupport, UltraLink™

Biosupport, UltraLink™

Supplier: Thermo Fisher Scientific

UltraLink™ Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilise proteins and other primary amine biomolecules for preparation of affinity purification columns.

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3M™ Harvest RC Single-Stage Chromatographic Purification Systems

3M™ Harvest RC Single-Stage Chromatographic Purification Systems

Supplier: 3M MEDICAL

Single-stage chromatographic purification for recombinant protein therapeutic manufacturing.

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Protein A Plus agarose, Pierce™

Protein A Plus agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein A Plus agarose is a high capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

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Protein A/G Plus agarose, Pierce™

Protein A/G Plus agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G plus agarose is a high capacity agarose resin for use in a variety of antibody affinity purification methods.

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Hydrazide resin, UltraLink™

Hydrazide resin, UltraLink™

Supplier: Thermo Fisher Scientific

UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

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