Supplier: Agilent

Agilent offers methods development kits to streamline the column collection process used for medium and high pressure LC systems.

Supplier: Merck

Ascentis® RP-Amide HPLC columns are optimised to the three terms of the resolution equation: efficiency, retention, and selectivity.

Supplier: Agilent

These HPLC columns are made with type A silica. They are useful for many applications of acidic or neutral compounds. These columns have a higher activity level and are therefore useful for separating isomers or other compounds where silanol activity enhances selectivity.

Supplier: Merck

BIOshell™ 400 Å Protein C18 columns contain 3,4 µm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of large proteins.

Supplier: Agilent

These cartridge columns are with reusable end fittings that have a unique design which makes them easy to install with finger-tight connections.

Supplier: Agilent

Eliminates particle leakage to improve data quality with light scattering detection. Typical applications: polyethylenes, polyolefins. Compatible with organic solvents.

Supplier: Merck

Discovery® BIO Wide Pore reversed-phase columns satisfy the need for efficiency, selectivity, scalability, and reproducibility for RP HPLC analyses of proteins, peptides, and small biomolecules.

Supplier: Agilent

Rugged, high-resolution separations up to five times faster than traditional 5 µm methods.

Supplier: Merck

BIOshell™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.

Supplier: Agilent

General purpose columns for solvents, sulfur compounds, oxygenated compounds, C1 to C3 isomers, methane, air/CO, and alkanes upto C12. Ideal for ethane, ethylene and ethyne separation.

Supplier: Merck

The LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar.

Supplier: Merck

Specially prepared and tested columns to seperate geometrical position (cis/trans) isomers of fatty acid methyl esters. Seperation of FAMEs should be take place in hydrogenated vegetable oil samples.

Supplier: Restek

These biphenyl columns offer a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases.

Supplier: Merck

These columns are situated between the traditional non bonded cyanosilicone phases SP-2330 and SP-2340 in polarity. The high polarity of this phase allows the separation of geometric (cis/trans) fatty acid methyl ester isomers as a group.

Supplier: Merck

Discovery® BIO Wide Pore reversed-phase columns satisfy the need for efficiency, selectivity, scalability, and reproducibility for RP HPLC analyses of proteins, peptides, and small biomolecules.

Supplier: Agilent

Bond Elut™ C18 is a hydrophobic silica sorbent. C18 is commonly used for non-polar compounds. Bond Elut™ C18 is endcapped to reduce interactions with silanol groups.

Supplier: Merck

Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.

Supplier: Restek

These columns are based on a wide pore material designed for optimal large-molecule separations. Base-deactivated, wide-pore packing exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C18 or Viva C8.

Supplier: Restek

Ultra amino columns are made of high-purity, spherical shaped type-B silica that minimises activity and creates high-density bonding. These columns are designed for selective and reliable HPLC applications. Aminopropylsilane provides reproducible retention and efficiency for normal-phase or HILIC analysis of simple sugars.

Supplier: Merck

SUPELCOGEL is a resin-based ion exclusion HPLC column which contains sulfonated divinylbenzene resins in six cationic forms. it is used for the unique selectivity for analyses of saccharides or organic acids. The SUPELCOGEL Ca columns are used to separate monosaccharides and sugar alcohols and also for separation of sugars in high fructose corn syrup.

Supplier: Restek

Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle® DB C18, but the shorter alkyl chain provides less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate. Replaces Hypersil® BDS C8 and Pinnacle® C8 Amine.

Supplier: Agilent

The Hypersil BDS is both base deactivated and encapped to improve peak shapes of basic solutes by minimising interaction with unreacted silanols. Hypersil ODS adds a monolayer of octadecyl silane (C18) to the hypersil silica support.

Supplier: Restek

Base-deactivated spherical silica that is optimised for UHPLC stability. Designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Complete resolution of all 16 EPA 610 PAHs, plus two other routinely analyzed PAH compounds, in less than five minutes to greatly reduce run times and increase sample throughput.

Supplier: Agilent

Poroshell 120 is made with a superficially porous particle that has a solid silica core and a porous outer layer. This particle provides high efficiency at lower pressures and is ideal for fast or high-resolution separations of many types of analytes.

Supplier: Restek

An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives.

Supplier: Restek

Rxi®-5Sil MS stationary phase incorporates phenyl groups in the polymer backbone. This improves thermal stability, reduces bleed, and makes the phase less prone to oxidation. These columns are ideal for GC-MS applications requiring high sensitivity, including use in ion trap systems.

Supplier: Restek

Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profile. It can effectively retain more types of solutes than a conventional C18 and is ideal for multicomponent LC-MS analyses. The general-purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions - even 100% aqueous. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time.

Supplier: Agilent

Poroshell 120 is made with a superficially porous particle that has a solid silica core and a porous outer layer. This particle provides high efficiency at lower pressures and is ideal for fast or high-resolution separations of many types of analytes.

Supplier: Restek

These biphenyl coulmns have a greater degree of dispersion than conventional phenyls and a greater degree of polarisability than phenyl hexyls, creating higher selectivity and a greater range of usability. With heightened interactions shows substantial increases in retention for dipolar, unsaturated, or conjugated solutes and enhanced orthogonal selectivity when using methanol mobile phases.

Supplier: Agilent

Step 2: Dispersive SPE cleanup
Select the dispersive SPE kit suited to the type of food being analysed and the method you are following. In this step, an aliquot of the sample extract from step one (see QuEChERS extraction kits) is added to a 2 or 15 ml centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning.