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HisPur™ cobalt superflow agarose, Pierce™

HisPur™ cobalt superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

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Monomeric avidin agarose, Pierce™

Monomeric avidin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

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Immobilised protein A, UltraLink™, Pierce™

Immobilised protein A, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

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Micro immobilisation kit, GlycoLink™

Supplier: Thermo Fisher Scientific

Hydrazide-activated resin to immobilise glycoproteins for large-scale affinity purification.

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Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Capacity Endotoxin Removal Resins are ideal for the removal of endotoxins from protein or antibody samples prior to cell transfection or animal injection.

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NHS-activated agarose, Pierce™

NHS-activated agarose, Pierce™

Supplier: Thermo Fisher Scientific

NHS-activated agarose is an amine-reactive, beaded-agarose resin for rapid and stable immobilisation of proteins, peptides and other ligands via primary amines.

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Immobilised Streptavidin resin, UltraLink™, Pierce™

Immobilised Streptavidin resin, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilised onto a beaded acrylamide support. UltraLink™ beads are hydrophilic, charge free, highly crosslinked, rigid and durable which makes the affinity resin suitable for a variety of batch or column type affinity procedures that require medium pressure chromatography.

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Ni-NTA His•Bind® Resin

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

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Control agarose resin, Pierce™

Control agarose resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.

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Ni-NTA His-Bind® Superflow™ Resin

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

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Spin purification kit for GST proteins, Pierce™

Spin purification kit for GST proteins, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ GST Spin Purification Kit provides a fast, easy to use format for purifying GST-fusion proteins from cellular lysates.

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Streptavidin agarose

Supplier: Merck Millipore (Novagen)

Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

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S-protein agarose, Novagen

Supplier: Merck Millipore (Novagen)

S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

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D-Desthiobiotin

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

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Immobilised β-cyclodextrin

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

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Novagen® GST Bind™ Resin, MilliporeSigma

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

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Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

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Protein G, MP Biomedicals

Supplier: MP Biomedicals

Protein G is a monomeric protein lacking cysteine residues. Recombinant Protein G is a highly stable surface receptor that is capable of binding the Fc portion of immunoglobulins, especially IgGs from a large number of species.

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Ion exchange chromatography media, Capto™ Q ImpRes

Ion exchange chromatography media, Capto™ Q ImpRes

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Sepharose Chromatography Resins, Strep-Tactin® XT

Sepharose Chromatography Resins, Strep-Tactin® XT

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin

Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin

Supplier: Cytiva

Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.

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Affinity chromatography media, IMAC Sepharose™ High Performance

Affinity chromatography media, IMAC Sepharose™ High Performance

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

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Affinity chromatography media, Capto™ Blue

Affinity chromatography media, Capto™ Blue

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

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Ion exchange chromatography media, Capto™ DEAE

Ion exchange chromatography media, Capto™ DEAE

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Gel filtration media, Sephadex™ G-25

Gel filtration media, Sephadex™ G-25

Supplier: Cytiva

Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

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Ion exchange chromatography media, SOURCE™ 30Q

Ion exchange chromatography media, SOURCE™ 30Q

Supplier: Cytiva

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Protease affinity chromatography media, PreScission™, Cytiva

Protease affinity chromatography media, PreScission™, Cytiva

Supplier: Cytiva

Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.

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Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.

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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media

Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

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