You searched for: Enzyme Assays

Supplier: AAT BIOQUEST

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.

Supplier: AAT BIOQUEST

Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.

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The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.

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Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

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Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.

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Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.

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The traditional BCA protein assay is widely used for quantifying proteins.

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Melanins have very diverse roles and functions in various organisms.

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Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

Supplier: AAT BIOQUEST

Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.

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Caspases play important roles in apoptosis and cell signaling.

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Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

Supplier: AAT BIOQUEST

Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.

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L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.

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Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

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Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Formaldehyde is a naturally occurring substance.

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Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.