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556 results for Cellular Assays

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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Black IHC chromogen (HRP), HIGHDEF®

Supplier: Enzo Life Sciences

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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CV-Caspase 3 & 7 Detection kit

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

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Apoptosis inducer set

Apoptosis inducer set

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

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Apoptosis inducer set

Supplier: Enzo Life Sciences

The set contains five reagents that induce apoptosis through different mechanisms.

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Red hydrogen peroxide assay kit

Supplier: Enzo Life Sciences

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

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ApoSENSOR™ Cell viability assay kit

Supplier: Enzo Life Sciences

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

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Cholesterol cell-based detection assay kit

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

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Solutions colorantes, Ghost Dye™

Solutions colorantes, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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Catalase fluorometric detection kit

Supplier: Enzo Life Sciences

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

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CFDA SE Cell proliferation assay kit

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

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Acetyltransferase activity kit

Supplier: Enzo Life Sciences

For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species.

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Nitric oxide reagents and kits

Nitric oxide reagents and kits

Supplier: Biotium

Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium

RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium

Supplier: Biotium

RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

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Blue IHC chromogen (HRP), HIGHDEF®

Supplier: Enzo Life Sciences

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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CV-Cathepsin L detection kit

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

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ROS-ID™ NO Detection kit

Supplier: Enzo Life Sciences

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

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FLUOR DE LYS® HDAC fluorometric activity assay kit

Supplier: Enzo Life Sciences

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

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Calcein-AM cell viability assay kits

Calcein-AM cell viability assay kits

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: Enzo Life Sciences

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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Green long-term cell tracer, CYTO-ID®

Supplier: Enzo Life Sciences

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

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Uric acid assay kit

Uric acid assay kit

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: Enzo Life Sciences

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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Yellow IHC chromogen (HRP), HighDef®

Supplier: Enzo Life Sciences

Yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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Red IHC chromogen (AP), HIGHDEF®

Supplier: Enzo Life Sciences

Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

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p70 S6K activity kit

Supplier: Enzo Life Sciences

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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qScript® ultra flex kits

qScript® ultra flex kits

Supplier: Quantabio

Faster, longer, tougher next gen cDNA synthesis with flexible priming.

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Cell proliferation assay kit, WST-1

Supplier: Cayman Chemical

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.

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p53 transcription factor assay kit

p53 transcription factor assay kit

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

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PPARδ transcription factor assay kit

PPARδ transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

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