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Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Steady-Luc™ Firefly HTS assay kit

Steady-Luc™ Firefly HTS assay kit

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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FluoForte™ Reagent

Supplier: Enzo Life Sciences

Calcium dye

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Live bacterial gram stain kit

Supplier: Biotium

Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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ExoBrite™ WGA EV Staining Kits

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.

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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81), Abcam

Supplier: Abcam

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.

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Catalase fluorometric detection kit

Supplier: Enzo Life Sciences

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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Fatty Acid Uptake Assay Kit Abcam

Supplier: Abcam

Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins.

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2-NBDG Glucose Uptake Assay Kit Abcam

Supplier: Abcam

2-NBDG Glucose Uptake Assay Kit ab287845 uses the fluorescent glucose analog 2-NBDG to quantify the rate of cellular glucose uptake. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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LipidLaunch™ BODIPY SM-102 LNP Kit (Loadable)

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.

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Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems

Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems

Supplier: CELL VIABILITY

The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.

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qScript® ultra flex kits

qScript® ultra flex kits

Supplier: Quantabio

Faster, longer, tougher next gen cDNA synthesis with flexible priming.

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ROS-ID™ superoxide detection kit

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

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CFDA SE Cell proliferation assay kit

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

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Green detection kit, LYSO-ID®

Supplier: Enzo Life Sciences

LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

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Steady membrane staining kits, CellBrite™

Steady membrane staining kits, CellBrite™

Supplier: Biotium

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

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