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Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Annexin V-FITC apoptosis detection kit II, Sigma-Aldrich®

Supplier: Merck Millipore (Oncogene)

Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.

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BCA protein assay kit

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

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Live-or-Dye NucFix™ red staining kit

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

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Yeast vitality staining kit

Yeast vitality staining kit

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Nrf2 transcription factor assay kit

Nrf2 transcription factor assay kit

Supplier: Cayman Chemical

Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.

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Red/green (FUN®1), ViaVac™

Red/green (FUN®1), ViaVac™

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Firefly luciferase assay kit

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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Live-or-Dye™ Yeast fixable live/dead staining kit

Live-or-Dye™ Yeast fixable live/dead staining kit

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

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CellBrite™ Fix membrane stains, Biotium

CellBrite™ Fix membrane stains, Biotium

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

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Cell proliferation assay kit, WST-1

Cell proliferation assay kit, WST-1

Supplier: Abnova

WST-1 cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model.

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Antioxidant assay kit

Antioxidant assay kit

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

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ATP assay kit

ATP assay kit

Supplier: Abnova

ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.

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Malachite green phosphate assay kit

Malachite green phosphate assay kit

Supplier: Abnova

Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.

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Pyruvate assay kit

Pyruvate assay kit

Supplier: Abnova

Pyruvate Assay Kit is for quantitative colorimetric/fluorimetric pyruvate determination.

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Nitric oxide assay kit

Nitric oxide assay kit

Supplier: Abnova

Nitric Oxide Assay Kit for the quantitative colorimetric determination of nitric oxide in cell, food sample, plasma, serum, tissue and urine.

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Methylated DNA capture kit

Supplier: Abnova

Methylated DNA Capture Kit is used to enrich methylated DNA.

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