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Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*

Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Phagocytosis is defined as the cellular uptake of particles within a plasma-membrane envelope by a cell.

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Cell Meter™ Fluorometric Intracellular Nitric Oxide (NO) Activity Assay Kit *NIR Fluorescence Optimized For Flow Cytometry*

Cell Meter™ Fluorometric Intracellular Nitric Oxide (NO) Activity Assay Kit *NIR Fluorescence Optimized For Flow Cytometry*

Supplier: AAT Bioquest

Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes.

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Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*

Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*

Supplier: AAT Bioquest

The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies.

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Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kits

Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kits

Supplier: AAT Bioquest

Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience.

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Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Red Fluorescence* (50 Tests)

Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Red Fluorescence* (50 Tests)

Supplier: AAT Bioquest

DNA fragmentation represents a characteristic of late stage apoptosis.

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Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*

Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

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Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*

Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*

Supplier: AAT Bioquest

Our Cell Explorer™ Live Cell Tracking Kit uses a proprietary orange fluorescent dye that gets enhanced fluorescence upon entering into live cells.

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Cell Meter™ Fluorescence Gap Junction Tracing Kit

Cell Meter™ Fluorescence Gap Junction Tracing Kit

Supplier: AAT Bioquest

Gap junctions are specialised intercellular membrane channels constituted with connexin that selectively facilitate the passage of small molecules of <1,5 Kd across cells.

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G-Biosciences Calcein Release Assay (Cell Viability Assay)

Supplier: G-Biosciences

Calcein AM Cell Viability Assay can be easily adapted to various fluorescence setups such as microplate assays, fluorescence microscope and flow cytometry. The assay is useful for various studies such as cell viability, cell adhesion, chemotaxis, multidrug resistance, apoptosis and cytotoxicity.

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BCA protein assay kit

Supplier: Merck Millipore (Novagen)

BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

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Firefly luciferase assay kit 2.0

Supplier: Biotium

This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.

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Amyloid plaque staining reagent, Amylo-Glo® RTD™

Amyloid plaque staining reagent, Amylo-Glo® RTD™

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

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Firefly and Renilla Luciferase single tube Assay

Firefly and Renilla Luciferase single tube Assay

Supplier: Biotium

This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.

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Alamar blue cell viability assay

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

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Yeast viability staining kits

Yeast viability staining kits

Supplier: Biotium

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

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Amyloid plaque stain reagent, with EtBr counter stain, Amylo-Glo® RTD™

Amyloid plaque stain reagent, with EtBr counter stain, Amylo-Glo® RTD™

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

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