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502 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Antibody labelling kits, Mix-n-Stain™ DNP

Supplier: Biotium

Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.

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Fluor-free acids, DyLight™

Fluor-free acids, DyLight™

Supplier: Thermo Fisher Scientific

DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

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Antibody labelling kits, Mix-n-Stain™ Digoxigenin

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.

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Streptavidin, maleimide-activated

Streptavidin, maleimide-activated

Supplier: Thermo Fisher Scientific

Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.

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Ghost Dye™ Red 710

Ghost Dye™ Red 710

Supplier: Tonbo Biosciences

Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.

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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Biotin NTA

Biotin NTA

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

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Reactive dye 5-packs, Amersham CyDye™

Reactive dye 5-packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.

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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

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Monofunctional maleimide fluors, CyDye™

Monofunctional maleimide fluors, CyDye™

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Amersham CyDye DIGE fluors

Amersham CyDye DIGE fluors

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

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Mono-Reactive NHS Ester dye packs, Amersham CyDye™

Mono-Reactive NHS Ester dye packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.

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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Supplier: Cytiva

Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.

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Amersham Cy3 and Cy5 Maleimide Mono-Reactive Dye 5-Packs, Cytiva

Amersham Cy3 and Cy5 Maleimide Mono-Reactive Dye 5-Packs, Cytiva

Supplier: Cytiva

Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Protein labelling Kit, Amersham™ QuickStain

Protein labelling Kit, Amersham™ QuickStain

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

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Fluorescent dyes, M series, streptavidin conjugate

Fluorescent dyes, M series, streptavidin conjugate

Supplier: Abnova

Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.

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Amersham™ CyDye™ Fluorescent Nucleotides

Amersham™ CyDye™ Fluorescent Nucleotides

Supplier: Cytiva

Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.

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CyDye Antibody Labeling Kits

CyDye Antibody Labeling Kits

Supplier: Cytiva

CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.

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Labeling dye, Atto 488, Sigma-Aldrich®

Supplier: Merck

Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.

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Peroxidase substrates, TrueBlue™

Supplier: SERACARE LIFE SCIENCES

KPL TrueBlue™ peroxidase substrate is a highly sensitive chromagenic substrate for visualisation of horseradish peroxidase-labeled reporter reagents.

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Atto 647N-biotin, BioReagent

Supplier: Merck

Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.

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DNA labeling reagent, Atto 647 NHS Ester

Supplier: Merck

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.

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