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You searched for: Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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MS-Compatible magnetic IP kit (Protein A/G), Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

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Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings)

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings)

Supplier: AAT BIOQUEST

Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

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Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.

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Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm.

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ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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ReadiLink™ Rapid Cy7 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy7.

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ReadiLink™ Rapid Cy3 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy3.

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Fluorescent dye removal columns, Pierce™

Fluorescent dye removal columns, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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ReadiLink™ Rapid iFluor® 750 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.

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Helixyte™ iFluor® 488 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 488 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 488 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Helixyte™ iFluor® 750 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 750 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 750 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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NHS-Azide and NHS-phosphine reagents, Pierce™

NHS-Azide and NHS-phosphine reagents, Pierce™

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

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ReadiLink™ xtra Rapid iFluor® 647 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 647 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

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iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

iFluor® 790 labelled WGA conjugate exhibits a distinct near-infrared fluorescence characteristic, emitting maximally at 812 nm.

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ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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ReadiLink™ Rapid mFluor™ Red 700 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ iFluor® 555 Oligo and ssDNA Labelling Kit

ReadiLink™ iFluor® 555 Oligo and ssDNA Labelling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Photoreactive biotinylation reagents, EZ-Link™

Photoreactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific

Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

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Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 594 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 350 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ BSA Conjugation Kit (2 Reactions)

ReadiLink™ BSA Conjugation Kit (2 Reactions)

Supplier: AAT BIOQUEST

Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

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ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Pierce™ Deuterium-labelled Cross Linkers

Pierce™ Deuterium-labelled Cross Linkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Supplier: AAT BIOQUEST

XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher.

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ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Streptavidin, hydrazide-activated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

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Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ NHS-Fluorescein antibody labeling kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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Magnetic RNA-protein pull-down kit, Pierce™

Magnetic RNA-protein pull-down kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Magnetic RNA-Protein Pull-Down Kit is a streamlined, robust method to enrich protein-RNA interactions using end-labelled RNA as the bait.

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